To make a good frozen section, ensure the quality of the section, and at the same time improve the accuracy of rapid pathological diagnosis during the operation, it has a great relationship with the understanding and selection of the frozen section machine.
The freezing slicer is composed of a slicing device and a refrigeration system. Generally, a complete freezing slicing system is composed of a host machine (freezer box, sample head, quick freezing table, etc.), a tool holder, and a blade. How to standardize the operation of the frozen slicer? Let's have a look
1. First, check whether the instrument is firmly and stably placed, whether the connection between the power plug and the socket is reliable, and whether the grounding is good. A certain gap is left around to facilitate ventilation and heat dissipation, and whether the voltage is normal.
2. Turn on the main switch of the power supply, check/set the temperature of the freezer, the temperature of the cold table, the temperature of the sample head, and the slice thickness, and enter the regular interface (generally, this machine is powered on for 24 hours, and is in the normal startup state, so as long as the cold table refrigeration is started at work and turned off at work).
3. Slicing procedure: put the "sampled" specimen with embedding agent on the sample head, place it on the quick freezing table for "quick freezing", lock the "slicing hand wheel". After a while, fix the frozen specimen with the sample clamp, repair the specimen first, then slice and patch.
4. Immerse the patch into "acetic alcohol solution" to "fix".
5. The cut samples shall be numbered and stored for standby or used in the downstream process.
6. Shutdown procedure - lock the hand wheel, clean the freezer, open the door of the freezer, and cut off the power switch. This machine is a continuous working machine, which is powered on continuously. Generally, it does not shut down. When it is not used for a short time, turn off the refrigeration of the cold table, and set the temperature of the freezer above 0 ℃ to stop.