Problems needing attention in making biological slides

(1) The quality of the materials will directly affect the quality of the slices. When taking materials, doctors should have a sharp material knife earlier, so as to avoid dragging the material knife back and forth, and it is better to cut in parallel with the fiber as much as possible when taking materials;

(2) Fixation is a part of the work of the technical room, and also an irremediable part of the whole production process. Fixation is to prevent tissue cells from autolysis and corruption, prevent the decomposition of proteins by enzymes in cells, and keep their original structure similar to life

(3) Water washing after fixation is usually ignored by many people, and incomplete washing is easy to cause poor color of the supporting plate and dyeing;

(4) Dehydration is to use a dehydrator to replace the water in the tissue, which is conducive to the penetration of organic solvents. Whether the dehydration is complete or not is directly related to whether the tissue can be fully transparent, and excessive dehydration is easy to cause brittle tissue;

(5) If you want to cut a slice well, you should have a sharp slicing knife earlier. Grinding knife is a relatively basic technology for slicing technicians. The quality of knife grinding is directly related to the quality of the slice;

(6) The dyeing time shall be determined according to the old and new fuel, temperature, and type of chips. The chips can be blued with warm water or tap water and washed fully to prevent fading caused by hydrochloric acid residues;

(7) The gum on the cover sheet shall not be too thin. When sealing the sheet, the gum shall be evenly filled with the cover sheet and the gum shall not overflow. The tissue shall be completely covered without bubbles and labeled. The label must be pasted on the left or right side of the slide. The number can be printed only after it is clearly written