1. Dehydration, transparency, too long wax soaking time and too high temperature may cause tissue brittleness. In addition, the texture of the tissue itself may also cause tissue brittleness. You can try to cut while blowing air to the wax slice, which may be relieved.
2. The knife is not sharp, the knife angle is too large, and the glass slide is too thick, which may cause the glass slide to roll up.
3. If the blade is uneven, the slide knife is not straight, and the slide knife is not parallel to the wax block, the wax may be bent.
4. If the knife is not clamped with the knife base and wax block, the thickness will be uneven if the tissue is too hard, and the slide machine spindle is too forward, or the slide machine has been worn.
5. The cracks on the glass slide may be caused by the knife gap, impurities in the paraffin, calcification, bone fragments or wired knots in the tissue, or cotton paper fibers.
6. Poor tissue dehydration may lead to the phenomenon that the glass slides are not connected, cannot be cut off, the glass slides are very thick, or the wax blocks behind the glass slides are white, invaginated, etc.
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