Paraffin section is also called permanent section, which is mainly suitable for the section of various plant tissues. The thickness of the section is easy to control, the clarity is good, and it can be made into continuous sections, which are easy to preserve for a long time. The manufacturer of paraffin slices tells you how to preserve plant paraffin slices.
Put the observation material in a specific fixative as soon as possible, kill its cells quickly, and try to keep it in its natural state of life. After the material is fixed, it needs to be preserved for use. In this process, the structure of the material is required not to change.
The commonly used preservation solvent is 70% alcohol solution, which can keep ordinary materials from getting bad for a long time. It is also required to be stored in the refrigerator.
Use of alcohol concentration: fix weak and tender plants with low concentration alcohol, such as 50%; Use 70% alcohol to fix older or harder materials.
If plant embryo materials are fixed, the formula can be modified to 50% alcohol 89ml+glacial acetic acid 5ml+formaldehyde 40% for storage
The four sides of the biological tissue slice are fixed without shrinkage and deformation, and the smooth surface of the plexiglass on both sides protects the two sides of the fixed slice, which is convenient for observing the internal structure of the biological tissue.
