Fluorescence microscope is a basic tool of immunofluorescence cytochemistry. It is composed of light source, filter plate system, optical system and other major components. Observe with ordinary optical microscope. Slide specimens are usually stained with hematoxylin eosin, sometimes with special staining.
First of all, the optical microscope should keep the natural state of the biological materials as far as possible to avoid artifacts, deformation and distortion, so the biological materials must be fixed; The film must be thin and transparent before it can be imaged under the optical microscope. In addition to cutting the material into thin slices or dispersing it by light pressing or other means, other methods must be used to make it transparent and dyed, so as to better observe the details of the structure.
In fact, all biological materials can obtain specimens that meet the requirements in thickness and transparency through paraffin embedding, slicing (or smearing), adhesion, wax dissolution, transparency and other processes, and then obtain a certain degree of contrast through dyeing procedures.
The biological slice is often pasted on the glass slide with a standard size of 26x76mm and a thickness of 1.1 ^ - 1.3mm. The two sides of the glass slide should be parallel planes. The requirement for the thickness of the glass slide is not very strict, but when its thickness exceeds the free working distance of some collectors with higher correction degree, the thickness of the glass slide becomes important for focusing the light source and forming the image of the field stop on the object plane. In this case, a glass slide with a thickness of 0.9 ^ - 1.0mm is appropriate.