Paraffin section is not only used to observe the morphological structure of normal cell tissue, but also the main method used by pathology and forensic science to study, observe and judge the morphological changes of cell tissue, but also has been widely used in the research of many other disciplines.
advantage:
1. It can cut (2 ~ 3 microns) thin sheets, which are thin and uniform without wrinkles.
2. The tissue structure is well preserved, the morphology of tissue cells is clear, the definition is very good, and can be made into continuous sections
3. The most outstanding advantage is that the slices can be preserved for a long time for teaching, scientific research, pathological diagnosis and reexamination, and the wax block can be used for retrospective research of other projects.
4. It is widely used and suitable for the production of various plant tissues
Disadvantages:
1. There are many procedures and links in paraffin production, and it takes several days to complete a cycle.
2. It makes the tissue smaller, the substances contained in the cells smaller, and the refraction is enhanced, which affects the accuracy of observation, and the cost is slightly higher
3. It is easy to cause the loss of antigenicity in the tissue during the production process, which affects the sensitivity of the results when used for immunohistochemical staining.
