The birth of pathological sections

1. Sampling: the fresher the tissue, the better. Generally, the human tissue is in vitro, and the animal tissue is quickly fixed after being killed to ensure the original morphological structure.

2. Fixation: small pieces of tissue removed from human or animal body must be immediately placed in liquid fixative for fixation.

3. Dehydration and transparency: after the specimen is fixed and washed, the tissue contains more water, and the water in the tissue block must be replaced. This process is called dehydration.

4. Wax dipping and Embedding:
(1) The paraffin is immersed in the tissue to replace the transparent agent contained in the tissue.
(2) Embedding: put the tissue soaked in wax into the melted solid paraffin. After the paraffin solidifies, the tissue is wrapped in it, which is called wax block. This process is called embedding.

5. Slice, patch and baking: after the slice is fully flattened on the constant temperature water surface, take the wax slice to the middle section of the slide, dump the residual water on the slide and remove the paraffin in the dissolved tissue gap.

6. Dyeing and sealing.