Tissue sampling is one of the important procedures for making slices. According to the specific requirements of teaching, scientific research and external inspection, the tissue is taken from human or animal, and the location and method of sampling are determined.
Specific requirements are as follows:
1. Fresh materials to ensure the original morphological structure
2. Size of tissue block The ideal volume of tissue block is 2.0x2.0x0.3cm, so that the fixative can rapidly and evenly penetrate into the tissue
3. Do not squeeze the tissue block. The blade of the tissue block should be sharp. Do not clamp the tissue too tightly to avoid deformation of the tissue and cells due to extrusion.
4. Standardize the sampling position. The sampling shall be accurate according to the anatomical position, and standardized according to the requirements.
5. Selecting the section of tissue block determines the direction of its section according to the structure of each tissue. Longitudinal and transverse sections are often the key to display the morphological structure of the tissue.
6. Keep the material clean. If there is dirt, mucus, etc. in the tissue block, it can be cleaned with water before being put into the fixing solution.
7. Keep the original shape of the tissue. After fixing the fresh tissue, most of the tissue will shrink, sometimes even completely deform. Therefore, flatten the tissue to maintain the prototype as much as possible.
