Paraffin section is the most widely used method in conventional histological techniques. It is not only used to observe the morphological structure of normal cells and tissues, but also the main method used by pathology, forensic medicine and other disciplines to study, observe and judge the morphological changes of cells and tissues, and has been widely used in many other disciplines and fields.
The paraffin sectioning method includes the steps of material extraction, fixation, washing and dehydration, transparency, wax immersion, embedding, sectioning and pasting, dewaxing, dyeing, dehydration, transparency and sealing.
Paraffin sections are usually used for HE staining or IHC. The thickness is thinner, generally 4um, the process is more complex, and the quality of the sample depends on the type and composition of different tissues, which is more dependent on the experience of the manufacturer. It needs to be constantly optimized in the experimental process to obtain the best experimental conditions.