Permanent slide specimens can be kept and used permanently.
Integral loading method:
It is a method to seal the whole tiny organism or part of the organ to make a slide specimen. This method is generally applicable to organisms or organs with small body or a thin body, such as unicellular algae, filamentous algae, fungi, soft bryophytes, protoplasts and sporangia of ferns, epidermis of higher plants, florets and pollen grains, protozoans, wings, antennae, feet of insects, and chick embryos
Attention shall be paid to:
(1) When holding the slide, it should be kept flat or placed on the platform. When dripping water, the amount of water should be appropriate, so as to be just covered by the cover glass.
(2) The materials shall be unfolded on the same plane without overlapping with dissecting needle or forceps.
(3) When placing the cover glass, slowly cover it on the water drop from one side to prevent bubbles.
(4) When dyeing, put a drop of staining liquid on one side of the cover glass, and suck it from the other side with absorbent paper to make the specimen under the cover glass uniformly colored. After coloring, use the same method, drop a drop of water, suck out the staining solution, and observe it under the microscope.
